PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid
SKU::RXSOL-60-6605-260
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PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid
Product Description:
PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic acid)) PIPES is a biological buffer (zwitterionic) designed by Good, et al. and typically referred to as Good's buffer useful in cell culture media formulations.
Product Application:
PIPES has two pKa values. One pKa (6.76 at 25°C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. The second pKa value is at 2.67 with a buffer range of from 1.5 - 3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.
Product Note:
Mild detergent extraction of chick brain synaptic plasma membranes followed by gel electrophoresis suggests that synaptic plasma membrane tubulin is an integral component. Although some of the synaptic plasma membrane tubulin might be aggregates, that possibility is not supported by the observation that tubulin aggregates that are added to synaptosomes before synaptic subfractionation do not partition with synaptic plasma membranes during membrane isolation.
Product Technical Specification:
Product alias:
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PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid</p>
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Product Delivery Locations:
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