WBC diluting fluid is used for perfoming the WBC count. Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber.
Normal saline should be used to make dilutions for total cell counts. Diluting fluids for WBC counts include: crystal violet/acetic acid. gentian violet/acetic acid.
Other Names
WBC DILUTING FLUID TRUCKS
Note
White cells can be similarly diluted by taking 0.05 ml of blood into 0.95 ml of white cell diluting fluid (R2) (this gives a 1 in 200 dilution).
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