TEMED (N,N,N',N'-Tetramethylethylenediamine) is a tertiary amine used to catalyze formation of free radicals from ammonium persulfate (APS) and riboflavin. Because of its properties, TEMED promotes the acrylamide polymerization process.
N,N,N',N'-tetramethylethylenediamine is an ethylenediamine derivative in which each nitrogen carries two methyl substituents. It is widely employed both as a ligand for metal ions and as a catalyst in organic polymerisation. It has a role as a chelator and a catalyst.
Other Names
NNNN-TETRAMETHYL ETHYLENEDIAMINE AR TEMED
Procedure
The reaction is a vinyl addition polymerization initiated by a free radical-generating system (Chrambach 1985). Polymerization is initiated by ammonium persulfate and TEMED (tetramethylethylenediamine): TEMED accelerates the rate of formation of free radicals from persulfate and these in turn catalyze polymerization.
Note
Thermo Scientific Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.
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