Kovacs reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde (DMAB), and concentrated hydrochloric acid. ... The indole produced is indicated by formation of a red coloured ring, soluble in ether, chloroform and alcohol.
Kovacs reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde (DMAB), and concentrated hydrochloric acid. ... The indole produced is indicated by formation of a red coloured ring, soluble in ether, chloroform and alcohol.
It is used for the diagnostic test, to determine the ability of the organism to split tryptophan into indole and alpha-aminopropionic acid by hydrolytic activity of bacteria that express tryptophanase enzyme (3).
Other Names
KOVACS INDOLE REAGENT
Procedure
Discrete bacterial colonies on solid medium. Tube method. 1% tryptophan or peptone broth. Kovác's reagent (for use with broth cultures).
Note
Remel Indole (Kovacs') is a reagent recommended for use in qualitative Previous tests for the detection of indole production required a xylene.
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