carbol fuchsin dilute staining solution identify Mycobacterium tuberculosis, the causative agent of tuberculosis. Acid-fast organisms have a lipoid capsule that has a high molecular weight and is waxy at room temperature.
Carbol fuchsin (0.3%) was prepared from 33 ml of the above solution diluted to 100 ml with distilled water before use.
Note
Ziehl-Neelsen stain is a useful differential staining to detect acid-alcohol resistant bacteria among
them the identification of mycobacteria, although it does not allow the differentiation of different
species. The acid-resistance characteristic of these microorganisms makes this coloration a rapid
method in the presumptive diagnosis of mycobacterial infection.
Fuchsin and retain color despite the action of acid and alcohol.Ziehl-Neelsen stain is a useful differential staining to detect acid-alcohol resistant bacteria among
them the identification of mycobacteria, although it does not allow the differentiation of different
species. The acid-resistance characteristic of these microorganisms makes this coloration a rapid
method in the presumptive diagnosis of mycobacterial infection. Mycobacteria are colored red by
Fuchsin and retain color despite the action of acid and alcohol.
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