BROMOPHENOL BLUE INDICATOR AR used as a laboratory indicator, changing from yellow below pH 3 to purple at pH and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis.
Other Names
BROMOPHENOL BLUE INDICATOR AR
Procedure
tetrabromophenolsulfonphthalein, BPB, albutest) is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid
Note
dyes with negative charge are bromophenol blue, orange G and xylene cyanol. The comb would be
placed near the end of the gel since DNA has a negative charge and will migrate towards the positive electrode only.
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