Brilliant cresyl blue solution is used in hematology as staining solution to examine reticulocytes in blood film.
Smear a small drop of the blood over the stain layer.
Other Names
BRILLIANT CRESYL BLUE FOR SOLUTION ALCHOHOLIC, BRILLIANT CRESYL BLUE
Procedure
Prepare a saturated solution of methylene blue by adding 1.5 g powdered methy- lene blue to 100 mL 95% ethyl alcohol.
Slowly add the alcohol to dissolve the powder
Note
Brilliant Cresyl Blue is a supravital stain used for staining reticulocytes and platelets.
It is a staining solution that makes target structures (by staining) in hematological specimen materials evaluable for diagnostic purposes
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